cd8 pe cy7 tonbo Search Results


cd8 pe cy7  (Cytek Biosciences)
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Cytek Biosciences cd8 pe cy7
Immunophenotype Results for Male and Female Sarcospan-Deficient Mice. Flow cytometry was used to determine the % immune cell populations in splenocyte suspensions obtained from WT and SSPN -/- male and female mice. In (A) T cells, (B) plasmacytoid dendritic cells (pDCs), (C) dendritic cells (DCs), and (G) natural killer NK1.1 + cells. Representative flow spectra are shown in (D) CD4+ and <t>CD8+</t> T cells (E) pDCs, and (F) DCs. Genotype differences in the male and female mouse groups were assessed separately using the two-tailed Student’s t-test. Data shown as individual values and considered significant if p ≥ 0.05. Significant differences indicated by * for 0.05 and ** for 0.001.
Cd8 Pe Cy7, supplied by Cytek Biosciences, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pe cy7 cd8  (Cytek Biosciences)
92
Cytek Biosciences pe cy7 cd8
Immunophenotype Results for Male and Female Sarcospan-Deficient Mice. Flow cytometry was used to determine the % immune cell populations in splenocyte suspensions obtained from WT and SSPN -/- male and female mice. In (A) T cells, (B) plasmacytoid dendritic cells (pDCs), (C) dendritic cells (DCs), and (G) natural killer NK1.1 + cells. Representative flow spectra are shown in (D) CD4+ and <t>CD8+</t> T cells (E) pDCs, and (F) DCs. Genotype differences in the male and female mouse groups were assessed separately using the two-tailed Student’s t-test. Data shown as individual values and considered significant if p ≥ 0.05. Significant differences indicated by * for 0.05 and ** for 0.001.
Pe Cy7 Cd8, supplied by Cytek Biosciences, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cd8-pe-cy7  (Cytek Biosciences)
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Immunophenotype Results for Male and Female Sarcospan-Deficient Mice. Flow cytometry was used to determine the % immune cell populations in splenocyte suspensions obtained from WT and SSPN -/- male and female mice. In (A) T cells, (B) plasmacytoid dendritic cells (pDCs), (C) dendritic cells (DCs), and (G) natural killer NK1.1 + cells. Representative flow spectra are shown in (D) CD4+ and <t>CD8+</t> T cells (E) pDCs, and (F) DCs. Genotype differences in the male and female mouse groups were assessed separately using the two-tailed Student’s t-test. Data shown as individual values and considered significant if p ≥ 0.05. Significant differences indicated by * for 0.05 and ** for 0.001.
Cd8 Pe Cy7, supplied by Cytek Biosciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cd4 pe cy7  (Cytek Biosciences)
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Immunophenotype Results for Male and Female Sarcospan-Deficient Mice. Flow cytometry was used to determine the % immune cell populations in splenocyte suspensions obtained from WT and SSPN -/- male and female mice. In (A) T cells, (B) plasmacytoid dendritic cells (pDCs), (C) dendritic cells (DCs), and (G) natural killer NK1.1 + cells. Representative flow spectra are shown in (D) CD4+ and <t>CD8+</t> T cells (E) pDCs, and (F) DCs. Genotype differences in the male and female mouse groups were assessed separately using the two-tailed Student’s t-test. Data shown as individual values and considered significant if p ≥ 0.05. Significant differences indicated by * for 0.05 and ** for 0.001.
Cd4 Pe Cy7, supplied by Cytek Biosciences, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pe cy7 anti cd8  (Cytek Biosciences)
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Cytek Biosciences pe cy7 anti cd8
Immunophenotype Results for Male and Female Sarcospan-Deficient Mice. Flow cytometry was used to determine the % immune cell populations in splenocyte suspensions obtained from WT and SSPN -/- male and female mice. In (A) T cells, (B) plasmacytoid dendritic cells (pDCs), (C) dendritic cells (DCs), and (G) natural killer NK1.1 + cells. Representative flow spectra are shown in (D) CD4+ and <t>CD8+</t> T cells (E) pDCs, and (F) DCs. Genotype differences in the male and female mouse groups were assessed separately using the two-tailed Student’s t-test. Data shown as individual values and considered significant if p ≥ 0.05. Significant differences indicated by * for 0.05 and ** for 0.001.
Pe Cy7 Anti Cd8, supplied by Cytek Biosciences, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti cd8 pe cy7  (Cytek Biosciences)
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Immunophenotype Results for Male and Female Sarcospan-Deficient Mice. Flow cytometry was used to determine the % immune cell populations in splenocyte suspensions obtained from WT and SSPN -/- male and female mice. In (A) T cells, (B) plasmacytoid dendritic cells (pDCs), (C) dendritic cells (DCs), and (G) natural killer NK1.1 + cells. Representative flow spectra are shown in (D) CD4+ and <t>CD8+</t> T cells (E) pDCs, and (F) DCs. Genotype differences in the male and female mouse groups were assessed separately using the two-tailed Student’s t-test. Data shown as individual values and considered significant if p ≥ 0.05. Significant differences indicated by * for 0.05 and ** for 0.001.
Anti Cd8 Pe Cy7, supplied by Cytek Biosciences, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cd8 pe cy7 rpa t8 tonbo biosciences  (Cytek Biosciences)
93
Cytek Biosciences cd8 pe cy7 rpa t8 tonbo biosciences
Immunophenotype Results for Male and Female Sarcospan-Deficient Mice. Flow cytometry was used to determine the % immune cell populations in splenocyte suspensions obtained from WT and SSPN -/- male and female mice. In (A) T cells, (B) plasmacytoid dendritic cells (pDCs), (C) dendritic cells (DCs), and (G) natural killer NK1.1 + cells. Representative flow spectra are shown in (D) CD4+ and <t>CD8+</t> T cells (E) pDCs, and (F) DCs. Genotype differences in the male and female mouse groups were assessed separately using the two-tailed Student’s t-test. Data shown as individual values and considered significant if p ≥ 0.05. Significant differences indicated by * for 0.05 and ** for 0.001.
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biolegend cd8 pe cy7  (Cytek Biosciences)
92
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Immunophenotype Results for Male and Female Sarcospan-Deficient Mice. Flow cytometry was used to determine the % immune cell populations in splenocyte suspensions obtained from WT and SSPN -/- male and female mice. In (A) T cells, (B) plasmacytoid dendritic cells (pDCs), (C) dendritic cells (DCs), and (G) natural killer NK1.1 + cells. Representative flow spectra are shown in (D) CD4+ and <t>CD8+</t> T cells (E) pDCs, and (F) DCs. Genotype differences in the male and female mouse groups were assessed separately using the two-tailed Student’s t-test. Data shown as individual values and considered significant if p ≥ 0.05. Significant differences indicated by * for 0.05 and ** for 0.001.
Biolegend Cd8 Pe Cy7, supplied by Cytek Biosciences, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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apc cy7 anti mouse cd8  (Cytek Biosciences)
93
Cytek Biosciences apc cy7 anti mouse cd8
Immunophenotype Results for Male and Female Sarcospan-Deficient Mice. Flow cytometry was used to determine the % immune cell populations in splenocyte suspensions obtained from WT and SSPN -/- male and female mice. In (A) T cells, (B) plasmacytoid dendritic cells (pDCs), (C) dendritic cells (DCs), and (G) natural killer NK1.1 + cells. Representative flow spectra are shown in (D) CD4+ and <t>CD8+</t> T cells (E) pDCs, and (F) DCs. Genotype differences in the male and female mouse groups were assessed separately using the two-tailed Student’s t-test. Data shown as individual values and considered significant if p ≥ 0.05. Significant differences indicated by * for 0.05 and ** for 0.001.
Apc Cy7 Anti Mouse Cd8, supplied by Cytek Biosciences, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cd8 apc cy7  (Cytek Biosciences)
93
Cytek Biosciences cd8 apc cy7
Immunophenotype Results for Male and Female Sarcospan-Deficient Mice. Flow cytometry was used to determine the % immune cell populations in splenocyte suspensions obtained from WT and SSPN -/- male and female mice. In (A) T cells, (B) plasmacytoid dendritic cells (pDCs), (C) dendritic cells (DCs), and (G) natural killer NK1.1 + cells. Representative flow spectra are shown in (D) CD4+ and <t>CD8+</t> T cells (E) pDCs, and (F) DCs. Genotype differences in the male and female mouse groups were assessed separately using the two-tailed Student’s t-test. Data shown as individual values and considered significant if p ≥ 0.05. Significant differences indicated by * for 0.05 and ** for 0.001.
Cd8 Apc Cy7, supplied by Cytek Biosciences, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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il2ra/cd25-apc  (Cytek Biosciences)
90
Cytek Biosciences il2ra/cd25-apc
Immunophenotype Results for Male and Female Sarcospan-Deficient Mice. Flow cytometry was used to determine the % immune cell populations in splenocyte suspensions obtained from WT and SSPN -/- male and female mice. In (A) T cells, (B) plasmacytoid dendritic cells (pDCs), (C) dendritic cells (DCs), and (G) natural killer NK1.1 + cells. Representative flow spectra are shown in (D) CD4+ and <t>CD8+</t> T cells (E) pDCs, and (F) DCs. Genotype differences in the male and female mouse groups were assessed separately using the two-tailed Student’s t-test. Data shown as individual values and considered significant if p ≥ 0.05. Significant differences indicated by * for 0.05 and ** for 0.001.
Il2ra/Cd25 Apc, supplied by Cytek Biosciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cd8 fitc  (Cytek Biosciences)
92
Cytek Biosciences cd8 fitc
FIGURE 3 | Comparison of intracellular cytotoxic molecules in resting CD103+ and <t>CD103−CD8+</t> T cells. (A) Representative dot plots and (B) graphs of intracellular levels of perforin, granzyme B (GZB) and granzyme A (GZA) in CD103+ and CD103−CD8+ T cells. (C) Comparison of intracellular levels of perforin, granzyme B (GZB) and granzyme A (GZA) in CD103+ and CD103−CD8+ T cells in the endometrium (EM), endocervix and ectocervix (CX/ECX). (D) Comparison of pre- vs. postmenopausal (white) women. Each dot represents a different patient. *p < 0.05, **p < 0.01, ***p < 0.001.
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Image Search Results


Immunophenotype Results for Male and Female Sarcospan-Deficient Mice. Flow cytometry was used to determine the % immune cell populations in splenocyte suspensions obtained from WT and SSPN -/- male and female mice. In (A) T cells, (B) plasmacytoid dendritic cells (pDCs), (C) dendritic cells (DCs), and (G) natural killer NK1.1 + cells. Representative flow spectra are shown in (D) CD4+ and CD8+ T cells (E) pDCs, and (F) DCs. Genotype differences in the male and female mouse groups were assessed separately using the two-tailed Student’s t-test. Data shown as individual values and considered significant if p ≥ 0.05. Significant differences indicated by * for 0.05 and ** for 0.001.

Journal: bioRxiv

Article Title: Assessing a Role for Sarcospan in Immune Function

doi: 10.1101/2024.03.31.587501

Figure Lengend Snippet: Immunophenotype Results for Male and Female Sarcospan-Deficient Mice. Flow cytometry was used to determine the % immune cell populations in splenocyte suspensions obtained from WT and SSPN -/- male and female mice. In (A) T cells, (B) plasmacytoid dendritic cells (pDCs), (C) dendritic cells (DCs), and (G) natural killer NK1.1 + cells. Representative flow spectra are shown in (D) CD4+ and CD8+ T cells (E) pDCs, and (F) DCs. Genotype differences in the male and female mouse groups were assessed separately using the two-tailed Student’s t-test. Data shown as individual values and considered significant if p ≥ 0.05. Significant differences indicated by * for 0.05 and ** for 0.001.

Article Snippet: Cells were incubated with anti-mouse primary antibodies to assess immune populations with the following conjugated antibodies: B220-APC (Tonbo Biosciences, San Diego, CA; clone RA3-6B2), CD19-PE/Dazzle594 (BioLegend, San Diego, CA; clone 6D5), CD3-AlexaFluor488 (BioLegend, clone 17A2), CD4-violetFluor450 (Tonbo Biosciences, clone GK1.5), CD8-PE/Cy7 (Tonbo Biosciences, clone 53-6.7), NK1.1-PE (Tonbo Biosciences, clone PK136), CD335/NKp46-APC (BioLegend, clone 29A1.4), CD11b-APC/Cy7 (Tonbo Biosciences, clone M1/70), CD11c-PerCp/Cy5.5 (Tonbo Biosciences, clone N418), Ly6G-PE (Tonbo Biosciences, clone RB6-8C5), and F4/80-FITC (Tonbo Biosciences, clone BM8.1,).

Techniques: Flow Cytometry, Two Tailed Test, IF-P

FIGURE 3 | Comparison of intracellular cytotoxic molecules in resting CD103+ and CD103−CD8+ T cells. (A) Representative dot plots and (B) graphs of intracellular levels of perforin, granzyme B (GZB) and granzyme A (GZA) in CD103+ and CD103−CD8+ T cells. (C) Comparison of intracellular levels of perforin, granzyme B (GZB) and granzyme A (GZA) in CD103+ and CD103−CD8+ T cells in the endometrium (EM), endocervix and ectocervix (CX/ECX). (D) Comparison of pre- vs. postmenopausal (white) women. Each dot represents a different patient. *p < 0.05, **p < 0.01, ***p < 0.001.

Journal: Frontiers in immunology

Article Title: Differential Cytotoxic Function of Resident and Non-resident CD8+ T Cells in the Human Female Reproductive Tract Before and After Menopause.

doi: 10.3389/fimmu.2020.01096

Figure Lengend Snippet: FIGURE 3 | Comparison of intracellular cytotoxic molecules in resting CD103+ and CD103−CD8+ T cells. (A) Representative dot plots and (B) graphs of intracellular levels of perforin, granzyme B (GZB) and granzyme A (GZA) in CD103+ and CD103−CD8+ T cells. (C) Comparison of intracellular levels of perforin, granzyme B (GZB) and granzyme A (GZA) in CD103+ and CD103−CD8+ T cells in the endometrium (EM), endocervix and ectocervix (CX/ECX). (D) Comparison of pre- vs. postmenopausal (white) women. Each dot represents a different patient. *p < 0.05, **p < 0.01, ***p < 0.001.

Article Snippet: Mixed cell suspensions were stained for surface markers with combinations of the following antibodies: CD45-vioblue 450, CD8-FITC (Tonbo), CD3-viogreen (Miltenyi), CD45-AF700, CD3-APC-Cy7, CD4-APC-Cy7, CD103–BV711 (Biolegend), CD4-PE-Cy5.5, CD103–PE-Cy7 (eBioscience, San Diego, CA), CD8-BUV395 (BD Bioscience).

Techniques: Comparison

FIGURE 4 | Comparison of degranulation and release of cytotoxic molecules in CD103+ and CD103−CD8+ T cells after activation. (A) Representative dot plot and (B) graph of CD107a expression in CD103+ and CD103−CD8+ T cells after activation. EM, CX, and ECX are shown combined. (C) Comparison of pre and postmenopausal women for CD107a expression. (D) Comparison of the percent of cells expressing perforin, granzyme B (GZB) and granzyme A (GZA) in CD103+ and CD103−CD8+ T cells from the endometrium (EM), endocervix and ectocervix (CX/ECX). Each dot represents a different patient. **p < 0.01, ***p < 0.001.

Journal: Frontiers in immunology

Article Title: Differential Cytotoxic Function of Resident and Non-resident CD8+ T Cells in the Human Female Reproductive Tract Before and After Menopause.

doi: 10.3389/fimmu.2020.01096

Figure Lengend Snippet: FIGURE 4 | Comparison of degranulation and release of cytotoxic molecules in CD103+ and CD103−CD8+ T cells after activation. (A) Representative dot plot and (B) graph of CD107a expression in CD103+ and CD103−CD8+ T cells after activation. EM, CX, and ECX are shown combined. (C) Comparison of pre and postmenopausal women for CD107a expression. (D) Comparison of the percent of cells expressing perforin, granzyme B (GZB) and granzyme A (GZA) in CD103+ and CD103−CD8+ T cells from the endometrium (EM), endocervix and ectocervix (CX/ECX). Each dot represents a different patient. **p < 0.01, ***p < 0.001.

Article Snippet: Mixed cell suspensions were stained for surface markers with combinations of the following antibodies: CD45-vioblue 450, CD8-FITC (Tonbo), CD3-viogreen (Miltenyi), CD45-AF700, CD3-APC-Cy7, CD4-APC-Cy7, CD103–BV711 (Biolegend), CD4-PE-Cy5.5, CD103–PE-Cy7 (eBioscience, San Diego, CA), CD8-BUV395 (BD Bioscience).

Techniques: Comparison, Activation Assay, Expressing

FIGURE 5 | Effect of menopausal status on release of cytotoxic molecules in CD103+ and CD103−CD8+ T cells after activation. (A) Comparison of the percent of cells expressing granzyme B (GZB), granzyme A (GZA), and perforin in CD103+ and CD103−CD8+ T cells from the endometrium (EM), endocervix and ectocervix (CX/ECX) in premenopausal and postmenopausal women. Each dot represents a different patient. (B) Pie chart representing the mean value of the percentage for all the patients shown in (A). (C) Changes in the ratio of granzyme A: granzyme B (GZA/GZB) in CD103+ and CD103−CD8+ T cells from the endometrium (EM), endocervix and ectocervix (CX/ECX) in premenopausal compared to postmenopausal women. *p < 0.05, **p < 0.01.

Journal: Frontiers in immunology

Article Title: Differential Cytotoxic Function of Resident and Non-resident CD8+ T Cells in the Human Female Reproductive Tract Before and After Menopause.

doi: 10.3389/fimmu.2020.01096

Figure Lengend Snippet: FIGURE 5 | Effect of menopausal status on release of cytotoxic molecules in CD103+ and CD103−CD8+ T cells after activation. (A) Comparison of the percent of cells expressing granzyme B (GZB), granzyme A (GZA), and perforin in CD103+ and CD103−CD8+ T cells from the endometrium (EM), endocervix and ectocervix (CX/ECX) in premenopausal and postmenopausal women. Each dot represents a different patient. (B) Pie chart representing the mean value of the percentage for all the patients shown in (A). (C) Changes in the ratio of granzyme A: granzyme B (GZA/GZB) in CD103+ and CD103−CD8+ T cells from the endometrium (EM), endocervix and ectocervix (CX/ECX) in premenopausal compared to postmenopausal women. *p < 0.05, **p < 0.01.

Article Snippet: Mixed cell suspensions were stained for surface markers with combinations of the following antibodies: CD45-vioblue 450, CD8-FITC (Tonbo), CD3-viogreen (Miltenyi), CD45-AF700, CD3-APC-Cy7, CD4-APC-Cy7, CD103–BV711 (Biolegend), CD4-PE-Cy5.5, CD103–PE-Cy7 (eBioscience, San Diego, CA), CD8-BUV395 (BD Bioscience).

Techniques: Activation Assay, Comparison, Expressing